载体用途：哺乳动物表达载体

原核抗性：Ampicillin（氨苄青霉素）

真核筛选标记：Zeocin（博来霉素）

启动子：CMV promter

表达水平：高

5' 测序引物：CMV-f

3' 测序引物：BGH-r

载体拷贝数：高

This pcDNA™3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin™ selectable marker and a forward-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family

Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli