Gene/insert name: cyan fluorescent protein
Backbone size w/o insert (bp): 5446
Cloning site 5': XhoI
Cloning site 3': XbaI
5' sequencing primer: T7
3' sequencing primer: GTCTTGTAGTTGCCGTCGTC
Selectable markers: Neomycin
The primer for sequencing out the 5' end of the GFP based constructs (GFP/EGFP, CFP, YFP) is 5'- GTCTTGTAGTTGCCGTCGTC -3'.