Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Promoter:EF-1α
Vector Type:pEF
Cloning Method:Restriction Enzyme/MCS
Protein Tag or Fusion:c-Myc Epitope Tag,His Tag (6x His)
Constitutive or Inducible System:Constitutive
pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements: The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.