Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS
Protein Tag or Fusion：V5 Epitope Tag，His Tag (6x His)
Constitutive or Inducible System：Constitutive

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

1. Human ubiquitin C promoter (hUbC) for high-level expression across a broad range of species and cell types (Schorpp et al., 1996; Wulff et al., 1990).
2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine (6×His) metal-binding tag.
3. Blasticidin resistance gene (bsd) for selection of stable cell lines.
4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.