载体用途：哺乳动物表达载体

原核抗性：Zeocin（博莱霉素）

真核筛选标记：Zeocin（博莱霉素）

启动子：CMV promter

标签：C端带V5标签及His标签

表达水平：高

5' 测序引物：CMV-f

3' 测序引物：BGH-r

载体拷贝数：高

GeneStorm Expression-Ready Clones are expressed from the vector pcDN3.1/GS. This vector utilizes the strong immediate-early cytomegalovirus (CMV) promoter for high-level, constitutive expression in mammalian cell lines. Each GeneStorm Clone is fused to a C-terminal peptide encoding the V5 epitope for detection with the Anti-V5-HRP Antibody and a 6His tag for purification on metal-chelating resin (i.e. ProBond). The vector also encodes the Zeocin resistance gene for selection in E. coli and for the  creation of stable mammalian cell lines.