载体用途：哺乳动物表达载体

载体别名：pcDNA3.1(-)-myc-His A、pcDNA3.1/myc-His(-) A

质粒大小：5522bp

原核抗性：Ampicillin（氨苄青霉素）

真核筛选标记：Neomycin / G418

启动子：CMV promter

标签：C端带myc标签及His标签

表达水平：高

5' 测序引物：CMV-f（通用测序引物，测序公司免费提供）

3' 测序引物：BGH-r（通用测序引物，测序公司免费提供）

载体拷贝数：高

最常用的哺乳动物表达载体之一。

CMV promoter强启动子调控外源基因表达，C端携带myc标签及His标签，方便进行表达检测。

载体拷贝数很高，表达量也很高。

无荧光标记。

Amp+原核筛选抗性，Neo+真核筛选抗性，可以利用G418筛选稳定细胞株。

pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.