载体用途:哺乳动物表达载体
原核抗性:Ampicillin(氨苄青霉素)
真核筛选标记:Zeocin(博莱霉素)
启动子:CMV promter
标签:C端带6xHis标签及Xpress Epitope标签
表达水平:高
5' 测序引物:CMV-f、T7
3' 测序引物:BGH-r
载体拷贝数:高
pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone. In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody. pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taqamplified PCR products.