载体用途：哺乳动物表达载体

原核抗性：Ampicillin（氨苄青霉素）

真核筛选标记：Neomycin / G418

启动子：CMV promter

标签：C端带V5标签及His标签

表达水平：高

5' 测序引物：CMV-f

3' 测序引物：BGH-r

载体拷贝数：高

        pcDNA ™ 3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA ™ 3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells.

        The vectors contain the following elements:

（1）Three reading frames to facilitate in-frame cloning with a C-terminal tag 

encoding the V5 epitope and a polyhistidine metal-binding peptide

（2）Human cytomegalovirus (CMV) immediate-early promoter for high-level 

expression in a wide range of mammalian cells

（3）Episomal replication in cell lines that are latently infected with SV40 or that 

express the SV40 large T antigen (e.g. COS7)

        The control plasmid, pcDNA ™ 3.1/V5-His/lacZ, contains a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. pcDNA ™ 3.1/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.